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大鼠去甲腎上腺素(NE)酶聯(lián)免疫吸附檢測(cè)試劑盒

ELK10884

規(guī)格：	價(jià)格：
48T	￥1960.00
96T	￥2800.00

下載說(shuō)明 ①

Overview 文獻(xiàn)

Product name:	Rat NE(Noradrenaline/Norepinephrine) ELISA Kit
Reactivity:	Rat
Alternative Names:	NA; NAd; Norepinephrine; Norepi
Assay Type:	Competitive Inhibition
Sensitivity:	26.2 pg/mL
Standard:	5000 pg/mL
Detection Range:	78.13-5000 pg/mL
Sample Type:	serum, plasma, cell lysates, cell culture supernates and other biological fluids
Assay Length:	2h
Research Area:	Signal transduction;Infection immunity;Neuro science;Hormone metabolism;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat NE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NE in the samples is then determined by comparing the OD of the samples to the standard curve.

Naunyn-Schmiedeberg's Archives of Pharmacology

Ginkgo biloba L. leaf extract (EGb 761) alleviates reserpine-induced depression-like symptoms in aged rats by enhancing serotonin/norepinephrine levels and reducing oxidative/nitrosative stress

日期：2025-04-17 IF：0

標(biāo)準(zhǔn)曲線(xiàn)

Concentration (pg/mL)	OD	Corrected OD
5000.00	0.175
2500.00	0.352
1250.00	0.655
625.00	0.856
312.50	1.225
156.25	1.596
78.13	1.813
0.00	2.211

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant NE and the recovery rates were calculated by comparing the measured value to the expected amount of NE in samples.

Matrix	Recovery range	Average
serum(n=5)	87-95%	91%
EDTA plasma(n=5)	93-107%	100%
Heparin plasma(n=5)	87-99%	93%

線(xiàn)性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.