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人核因子κB亞基p65(NFκB-p65)酶聯(lián)免疫吸附檢測試劑盒

ELK9589

規(guī)格：	價格：
48T	￥1960.00
96T	￥2800.00

下載說明 ①

Overview 文獻

Product name:	Human NFκB-p65(Nuclear Factor Kappa B p65) ELISA Kit
Reactivity:	Human
Alternative Names:	NFKB3; p65; RelA
Assay Type:	Sandwich
Sensitivity:	0.1 ng/mL
Standard:	10 ng/mL
Detection Range:	0.16-10 ng/mL
Sample Type:	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length:	3.5h
Research Area:	Signal transduction;
Uniprot ID:	Q04206
Test principle:	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NFκB-p65. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NFκB-p65. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NFκB-p65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NFκB-p65 in the samples is then determined by comparing the OD of the samples to the standard curve.

International Journal of Molecular Sciences

The Impact of Recreational Diving to a Depth of 40 m on Selected Intracellular Damps

日期：2025-04-17 IF：4.9

標準曲線

Concentration (ng/mL)	OD	Corrected OD
10.00	1.956	1.858
5.00	1.674	1.576
2.50	1.174	1.076
1.25	0.797	0.699
0.63	0.555	0.457
0.32	0.377	0.279
0.16	0.236	0.138
0.00	0.098	0.000

精密度

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

回收率

Matrices listed below were spiked with certain level of recombinant NFκB-p65 and the recovery rates were calculated by comparing the measured value to the expected amount of NFκB-p65 in samples.

Matrix	Recovery range	Average
serum(n=5)	96-107%	101%
EDTA plasma(n=5)	79-93%	86%
Heparin plasma(n=5)	80-97%	88%

線性

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NFκB-p65 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.