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Cdc2 (phospho Thr161) rabbit pAb

ES1280

規(guī)格：	價格：
50μL	￥1280.00
100μL	￥1980.00

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Overview

Product name:	Cdc2 (phospho Thr161) rabbit pAb
Reactivity:	Human;Mouse;Rat
Alternative Names:	CDK1; CDC2; CDC28A; CDKN1; P34CDC2; Cyclin-dependent kinase 1; CDK1; Cell division control protein 2 homolog; Cell division protein kinase 1; p34 protein kinase
Source:	Rabbit
Dilutions:	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Immunogen:	The antiserum was produced against synthesized peptide derived from human CDC2 around the phosphorylation site of Thr161. AA range:131-180
Storage:	-20°C/1 year
Clonality:	Polyclonal
Isotype:	IgG
Concentration:	1 mg/ml
Observed Band:	34kD
GeneID:	983
Human Swiss-Prot No:	P06493
Cellular localization:	Nucleus. Cytoplasm. Mitochondrion . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle. Cytoplasmic during the interphase. Colocalizes with SIRT2 on centrosome during prophase and on splindle fibers during metaphase of the mitotic cell cycle. Reversibly translocated from cytoplasm to nucleus when phosphorylated before G2-M transition when associated with cyclin-B1. Accumulates in mitochondria in G2-arrested cells upon DNA-damage.
Background:	cyclin dependent kinase 1(CDK1) Homo sapiens The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009],

Western Blot analysis of various cells using Phospho-Cdc2 (T161) Polyclonal Antibody diluted at 1:1000
Western Blot analysis of HeLa cells using Phospho-Cdc2 (T161) Polyclonal Antibody diluted at 1:1000
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using CDC2 (Phospho-Thr161) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from HeLa cells, using CDC2 (Phospho-Thr161) Antibody. The lane on the right is blocked with the phospho peptide.

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